...iorat\data\papers\10022891.txtDIPinteraction1 34.42%STA1 STA1 under STA1 promoter function-inducing conditions and wasconsidered a candidate for the multicopy inhibitor of STA1
...iorat\data\papers\10022891.txtDIPinteraction1 37.11%MSS1 glucoamylase Interestingly, the NRG1 gene has previously been identifiedas MSS1 in a genetic screen for a multicopy suppressor of elevated glucoamylase production caused by sns1 mutation (1).The fact that the same gene was isolated by two different approaches to identify negative factors for STA1 expressionimplies that the NRG1 gene may play an important role in the negative regulation process.Nrg1 is involved in glucose repression of STA1 gene expression.
...iorat\data\papers\10022891.txtDIPinteraction1ba 54.71%nrg STA1 nrg1D alleviates glucose repression of STA1 transcription.
...iorat\data\papers\10022891.txtDIPinteraction3 60.81%Nrg1 Ssn6 These two-hybrid results, together with the genetic interac-tion between Nrg1 and Ssn6-Tup1, suggested the possibility of direct interaction between Nrg1 and Ssn6.
...iorat\data\papers\10022891.txtDIPinteraction1 68.98%major nrg1 Although we cannot exclude the possibility of the in-volvement of Mig1, Mig2, and/or some unknown repressors, we suggest that Nrg1 is the major repressor responsible for theglucose repression of the STA genes since nrg1
...iorat\data\papers\10022891.txtDIPinteraction1ba 4.88%Snf1 Mig1 In theabsence of glucose, the Snf1 kinase inhibits the function of Mig1 protein directly or indirectly, leading to derepression ofglucose-repressed genes (3, 4).
...iorat\data\papers\10022891.txtDIPinteraction3 67.32%Nrg1 Ssn6 hybrid and GST pull-down experiments demonstrate the phys-ical interaction between Nrg1 and Ssn6 both in vivo and in vitro.Nrg1 joins Mig1 and Mig2 as a DNA-binding repressor for glucose repression.
...iorat\data\papers\10022891.txtDIPinteraction1b 83.13%STA1 MAT Regulation of STA1 gene expression by MAT during thelife cycle of Saccharomyces cerevisiae.
...iorat\data\papers\10022891.txtDIPinteraction1b 59.54%Nrg1 Ssn6 Nrg1 interacts with Ssn6 both in vivo and in vitro.
...iorat\data\papers\10022891.txtDIPinteraction1ba 38.24%Nrg1 STA1 STA-TATASTA-TPK2-integrated cells grown inglucose (data not shown), it is possible that Nrg1 mediates
...iorat\data\papers\10022891.txtDIPinteraction1b 77.31%Nrg1 Ssn6 Nrg1 interacts with Ssn6.
...iorat\data\papers\10022891.txtDIPinteraction2 3.1%Nrg1 Ssn6 Two-hybrid and glutathione S-transferase pull-down experiments show an interaction of Nrg1 with Ssn6 both in vivo and in vitro.
...iorat\data\papers\10022891.txtDIPinteraction2 60.03%Nrg1 Ssn6 The interaction of Nrg1 with Ssn6 was confirmed by another two-hybridassay (Fig. 7A).
...iorat\data\papers\10022891.txtDIPinteraction1 69.63%mig1 glucoamylase this notion are our observations that the STA promoters do notcontain a consensus Mig1-binding sequence and mig1
...iorat\data\papers\10022891.txtDIPinteraction1 3.38%Nrg1 STA1 These findings indicate that Nrg1 acts as a DNA-binding repressor and mediatesglucose repression of the STA1 gene expression by recruiting the Ssn6-Tup1 complex.
...iorat\data\papers\10022891.txtDIPinteraction1ba 39.92%Nrg1 STA1 Nrg1 relieves glucose repression of STA1 gene expression.Northern blot analysis was performed to determine whether the increased glucoamylase activity in nrg1D cells correlateswith the transcription level of the STA1 gene.
...iorat\data\papers\10022891.txtDIPinteraction1 63.47%glucoamylase nrg1 Second, Northern analyses show that the increased glucoamylase level iscorrelated with the increased level of STA1 transcript in nrg1
...iorat\data\papers\10022891.txtDIPinteraction1 39.49%nrg1 glucoamylase In contrast, nrg1
...iorat\data\papers\10022891.txtDIPinteraction1 41.37%Nrg1 STA1 Taken together, the results of the glucoamylase assay and Northernanalysis indicate that Nrg1 is required for glucose repression of STA1 gene expression.Nrg1 binds to an upstream element of the STA1 promoter.
...iorat\data\papers\10022891.txtDIPinteraction1b 62.04%Nrg1 Ssn6 The results revealed that Nrg1 interacts with Ssn6 in vitro (Fig. 7B).
...iorat\data\papers\10022891.txtDIPinteraction1 97.1%Ssn6 a2 The tetratricopeptiderepeats of Ssn6 interacts with the homeo domain of
...iorat\data\papers\10022891.txtDIPinteraction1ba 38.96%Glucoamylase nrg1 Glucoamylase production in wild-type and nrg1D cells was monitored during growthin minimal medium containing glucose as the sole carbon source.
...iorat\data\papers\10022891.txtDIPinteraction2 62.36%Nrg1 Ssn6 The results from the two-hybrid and GST pull-down analyses, therefore, indicatethe physical interaction of Nrg1 with Ssn6 both in vivo and in vitro.
...iorat\data\papers\10022891.txtDIPinteraction2 27.09%Nrg1 Ssn6 In vivo interaction of Nrg1 with Ssn6 was tested with the plasmids expressingDBD-Nrg1 and AD-Ssn6 (Ssn6 residues 1 to 403 fused with the Gal4 AD) (48) and strain Y190 (12).
...iorat\data\papers\10022891.txtDIPinteraction1ba 34.18%glucoamylase Sta1 STA-TATASTA-TPK2 (Fig. 1B) and significantly de-creased glucoamylase production to the Sta1 strain (Table 2)
...iorat\data\papers\10022891.txtDIPinteraction1 62.68%Nrg1 STA1 DISCUSSION We provide here several lines of evidence for the identifi-cation of Nrg1 as a transcriptional repressor responsible for
...iorat\data\papers\10022891.txtDIPinteraction1b 39.92%glucoamylase nrg1 Nrg1 relieves glucose repression of STA1 gene expression.Northern blot analysis was performed to determine whether the increased glucoamylase activity in nrg1D cells correlateswith the transcription level of the STA1 gene.
...iorat\data\papers\10024454.txtDIPinteraction1 40.04%PSAT AMG Since Arg42 and Arg77 of PSAT both take
...iorat\data\papers\10024454.txtDIPinteraction6 44.57%PSAT AAT A comparison of substrate analogue complexes of PSAT and AAT
...iorat\data\papers\10024454.txtDIPinteraction1 30.49%PLP PLP Hydrogen-bonding of PLP and PLP-AMG to PSAT
...iorat\data\papers\10024454.txtDIPinteraction1b 63.66%nine AMG A third argi- nine binds the AMG side-chain indirectly through a solvent molecule but is expected to position itself during catalysis between the PLP phosphate group and the substrate side-chain.
...iorat\data\papers\10024454.txtDIPinteraction1ba 90.79%la des dans la deA^termination des structures cristallines.
...iorat\data\papers\10024454.txtDIPinteraction1 45.36%a3 PLP The most rel- evant structural differences responsible for this are the already mentioned shift of a-helix 5 of AAT- AMA with respect to helix a3 of PSAT and the hydrogen bonding of Arg266 of AAT-AMA with the PLP phosphate group (Figures 4(a), 5(a) and 7(c); JaE` ger et al., 1994).
...iorat\data\papers\10024454.txtDIPinteraction1ba 62.88%PSAT AMG Analysis of the active site of PSAT and of the complex with AMG demonstrates that the enzyme is well adapted for binding a highly negatively charged substrate side-chain.
...iorat\data\papers\10024454.txtDIPinteraction4 15.65%area PSAT The total accessible surface area of the PSAT dimer is 26,667 AE^ 2 when a probe
...iorat\data\papers\10024454.txtDIPinteraction1 36.07%PLP PLP One solvent molecule mediates hydrogen bonds of Gly10 and Thr240 to PLP while a second mediates a hydrogen bond of Arg77 to PLP.
...iorat\data\papers\10024454.txtDIPinteraction1 48.1%PSAT PLP In the unligated state of PSAT no hydrogen bond to atom O30 of PLP is made.
...iorat\data\papers\10024454.txtDIPinteraction2 86.2%ase pH kinetics of the reaction of aspartate aminotransfer- ase with aspartate at low pH reveals dual routes in the enzyme substrate association process.
...iorat\data\papers\10026184.txtDIPinteraction4 13.62%RNA polymerase II holoenzyme Nonetheless, BRCA1 has been reported associated with certain chromatin structures in vivo and linked to the RNA polymerase II holoenzyme complex (15, 16).
...iorat\data\papers\10026184.txtDIPinteraction4 26.37%BARD1 BRCA1 BARD1 homodimer and BRCA1/BARD1 heterodimer sample (1.0 ml)concentrations were adjusted to 1
...iorat\data\papers\10026184.txtDIPinteraction1 0%BRCA1 BARD1 Mapping the Functional Domains of BRCA1 INTERACTION OF THE RING FINGER DOMAINS OF BRCA1 AND BARD1*
...iorat\data\papers\10026184.txtDIPinteraction4 78.54%BRCA1 BARD1 Proteolysis mapping and fluorescence experiments provide general structural insights as a preliminary step toward building a picture of the BRCA1/BARD1 heterodimer.
...iorat\data\papers\10026184.txtDIPinteraction4 82.28%BRCA1 BARD1 BRCA1 and BARD1 N-terminal complexes do not bindnucleic acids.
...iorat\data\papers\10026191.txtDIPinteraction1b 0%II Live Transforming Growth Factor-b Induces Formation of aDithiothreitol-resistant Type I/Type II Receptor Complex in Live Cells*
...iorat\data\papers\10026191.txtDIPinteraction1ba 54.49%step PAI-1 DTT Pretreatment Prevents TGF-b-induced Activation of the Type I Receptor--In epithelial cells, an essential step in TGFb-mediated growth inhibition and PAI-1 promoter activation is activation of the ability of TbRI to rapidly phosphorylate Smad3 at its C-terminal SSVS motif (28).
...iorat\data\papers\10026191.txtDIPinteractionZZZ: > fold 'VB' Smad3 'NNP'< 59.34%fold Smad3 TGF-b Receptor Complexes 5719 20-fold increase in Smad3 phosphorylation; two-dimensional tryptic mapping indicated that this in vitro phophorylation occurred at the same site as in vivo (data not shown).
...iorat\data\papers\10026196.txtDIPinteraction1ba 69.76%Fc BAF TABLE IISummary of complex formation and BAF data for biotinylated ligands Biotinylatedligand Location ofmutation Ratio IL-11R-Fc binding Ratio complex formationa Ratio BAF stimulation
...iorat\data\papers\10026196.txtDIPinteraction2 50.52%II gp130- This allowed us to examine the binding of these site II mutants to gp130-Fc (in the presence of soluble IL-11R), which we were unable to do by measuring competitive binding between bIL-11 and the mutants, as the mutant proteins were still able to compete for soluble IL-11R.
...iorat\data\papers\10026196.txtDIPinteraction1 22.21%gp130 pIG Dot-blot analysis showed that both monoclonal antibodies preferentially recognized native mIL-11 and not de-natured mIL-11, indicating that their reactivity with mIL-11 is conformation-dependent.IL-11R-Fc and gp130-Fc Expression Constructs--The construction of the eukaryotic expression plasmids pIG/IL-11R-Fc and pIG/gp130-Fchas been described previously (17).
...iorat\data\papers\10026196.txtDIPinteraction1b 52.32%Fc BAF TABLE ISummary of receptor binding and BAF assay data for mIL-11 mutants Wild type or mIL-11mutant Location ofmutationa Ratio IL-11R-Fc binding Ratio BAF stimulation
...iorat\data\papers\10026196.txtDIPinteraction3 7.77%IL-11 gp130 IL-11 has been shown to function in a similar manner, and, as for IL-6, a ligand-specific IL-11 receptor (IL11R) (15, 16) functions to promote the formation of a high affinity complex between IL-11 and gp130 (17).
...iorat\data\papers\10026196.txtDIPinteraction1ba 79.6%IL-6 gp130 Site III enables IL-6 to bind to a second gp130 molecule and form hexameric complexes (29), and site III of LIF enables it to bind to the LIF receptor (32).
...iorat\data\papers\10026196.txtDIPinteraction1ba 62.94%IL-11 gp130 A complex of IL-11 and the IL-11R interacts with gp130 to induce this homodimerization.
...iorat\data\papers\10026196.txtDIPinteraction6 1.4%IL-11 IL-11 A complex of IL-11 and the IL-11 receptor(IL-11R) has been shown to interact with gp130, with high affinity, and to induce gp130- dependent signaling.In this study, we have identified residues crucial for the binding of murine IL-11 (mIL-11) to both the IL-11R andgp130 by examining the activities of mIL-11 mutants in receptor binding and cell proliferation assays.
...iorat\data\papers\10026196.txtDIPinteractionZZZ: > fold 'VB' BAF 'NNP'< 52.12%fold BAF This biotinylated mutant bound to IL-11R-Fc with a 5-fold increase in affinity
...iorat\data\papers\10026196.txtDIPinteractionZZZ: > protease 'NN' a 'DT' protein 'NN'< 18.5%protease a protein Cleavage of the glutathione S-transferasemIL-11 fusion protein with 3C protease produces a protein consisting ofamino acids 1-178 of mIL-11 with an extra glycine at the NH
...iorat\data\papers\10026196.txtDIPinteractionZZZ: > Fc 'NNP' F 'NN' 3 'CD'< 45.37%Fc F3 Activity of Site II Mutants--The activities of the site II mutants were assessed in both the IL-11R-Fc binding assay and the Ba/F3-mgp130/mIL-11R proliferation assay (Table I and
...iorat\data\papers\10026196.txtDIPinteractionZZZ: > Fc 'NNP' F 'NN' 3 'CD'< 55.3%Fc F3 Activity of Site III Mutants--The activities of the site III mutants W147A and R151A were assessed in the IL-11R-Fc binding assay and the Ba/F3-mgp130-mIL-11R proliferation assay (Table I and Figs.
...iorat\data\papers\10026281.txtDIPinteraction4 84.97%plastocyanin cytochrome The results are similar to the effects of ionic strength on the reduction of the positivelycharged cytochrome c and the negatively charged plastocyanin by the cytochrome bf complex.
...iorat\data\papers\10026281.txtDIPinteraction1ba 1.66%AMP ETF Close analysis of the structure reveals that the loop containing a^I63 isin part responsible for conferring the high specificity of AMP binding by the ETF protein.
...iorat\data\papers\10026281.txtDIPinteraction1 88.01%ETF ETF-QO 7, 1999 1987 denitrificans and human ETF semiquinones as substrates ofpig ETF-QO in the disproportionation reaction catalyzed by
...iorat\data\papers\10026281.txtDIPinteraction3 11.99%ETF ETF-QO Previously, a chimeric human-Paracoccus ETF protein was constructedin which the first 84 residues of the human a^-subunit were substituted by those of the P. denitrificans sequence (19).While this region still retains 64% sequence identity with the original human ETF protein, this chimeric ETF was foundto be markedly diminished in the reaction between ETF and ETF-QO, suggesting that residues within the N-terminalportion of the a^-subunit are necessary for interactions with ETF-QO (19).
...iorat\data\papers\10026281.txtDIPinteraction1ba 63.2%AMP thea AMP binds ETF exclusively within thea^-subunit, with residues in the binding site being contributed by those within strand 1, at the C-terminal end of strand 2,and from strands 4, 4a, and 4b (see Figure 3B).
...iorat\data\papers\10026281.txtDIPinteraction3 28.34%ETFs pig The reaction between the ETFs and pig ETFQO was assayed by the catalyzed disproportionation ETFsemiquinone reaction (35).
...iorat\data\papers\10026281.txtDIPinteraction4 85.27%plastocyanin cytochrome However, in thereaction of plastocyanin and the cytochrome bf complex, the results are apparently due to local charges on the redoxproteins in the electron transfer complex and therefore shortrange forces (47).The original description of P. denitrificans ETF indicated that the bacterial ETF did not reduce mammalian (pig) ETF-QO when assayed as a ubiquinone reductase (3).
...iorat\data\papers\10026281.txtDIPinteraction2 83.15%MCAD ETF Figure 7 comparesthe ionic strength dependence of the reaction of MCAD with human ETF, P. denitrificans ETF, and a human-Paracoccuschimeric ETF (19); all reactions were conducted at 1 uM ETF at pH 8.0, the pH optimum for the dehydrogenase.
...iorat\data\papers\10048790.txtDIPinteraction3 3.27%Cdc25 adenylyl cyclase These studies suggest that a direct interaction between Cdc25 and adenylyl cyclase promotes efficient assembly of the adenylyl cyclase complex.
...iorat\data\papers\10048790.txtDIPinteraction1b 31.91%Cdc25p cAMP SDS-PAGE gels were transferred to Cdc25p Decrease the cAMP Response to Glucose nitrocellulose membranes or stained with Coomassie blue.
...iorat\data\papers\10048790.txtDIPinteraction1 68.63%Ras adenylyl cyclase Ras and SH3 do-mains cause synergistic activation of adenylyl cyclase activity.Precipitations were carried out by using soluble membrane ex-tracts prepared from strain JF36 (ras1, ras2) [32].
...iorat\data\papers\10048790.txtDIPinteraction1ba 40.26%map Cdc25p These dataThe cAMP measurements suggested that Cdc25p SH3 suggest that Cdc25-SH3 binds directly to adenylyl cyclase.binds directly to other components of the Ras signalling sysTo further map the region bound by Cdc25p1-134, wetem.
...iorat\data\papers\10048790.txtDIPinteraction3 72.34%Cdc25 adenylyl cyclase Cdc25 may prefer non-PXXP binding partners.binding site is in the C-terminal region of adenylyl cyclase near the centre of the catalytic region, a region where there Several lines of evidence suggest that the interaction between Cdc25 and adenylyl cyclase is a bonafide SH3 inter-are no PXXP consensus binding-site motifs.
...iorat\data\papers\10048790.txtDIPinteraction1ba 74.48%adenylyl cyclase Cap Grb2 also binds Cap, but Grb2 has two SH3 sequently does not associate with the membrane is nonfunctional when expressed from the chromosome but isdomains; perhaps one binds adenylyl cyclase and the other binds Cap.
...iorat\data\papers\10048790.txtDIPinteraction1 0.43%Cdc25p cAMP cerevisiae Cdc25p Binds Adenylyl Cyclase and Facilitates Ras Regulation of cAMP Signalling
...iorat\data\papers\10048790.txtDIPinteraction1 25.83%Protein GRF SH3SF9 Cell Protein Expression and Extract Preparation domain and Ras GRF domain are shown.
...iorat\data\papers\10048790.txtDIPinteraction1b 51.68%Cyclase Ras Cyclase Activity by Ras and SH3 Domains
...iorat\data\papers\10048790.txtDIPinteraction1 73.31%Cdc25 Cap First, Cdc25 SH3 binds to cyclase and not Cap,
...iorat\data\papers\10048790.txtDIPinteraction1b 36.77%Cdc25p cAMP Mutations in theSH3 domain of Cdc25p decrease the cAMP response to glucose.Glucose-starved yeast cells were given glucose to a final concen-tration of 25 mM.
...iorat\data\papers\10048790.txtDIPinteraction2 11.94%Grb2 GST expression of Grb2 as a fusion to GST in E. coli (GST-activate Ras [3].
...iorat\data\papers\10048790.txtDIPinteraction1b 74.48%Grb2 Cap Grb2 also binds Cap, but Grb2 has two SH3 sequently does not associate with the membrane is nonfunctional when expressed from the chromosome but isdomains; perhaps one binds adenylyl cyclase and the other binds Cap.
...iorat\data\papers\10048790.txtDIPinteraction1b 63.94%Ras adenylyl cyclase The binding accelerated Ras activation of adenylyl cyclase in vitro.
...iorat\data\papers\10048790.txtDIPinteraction1b 5.36%Ras GRF GTP-bound Ras binds Ras-GRF, which is activated by Ca21 [11].and activates downstream effectors such as Raf, a protein kiIn yeast, Cdc25p is the Ras GEF [12-15].
...iorat\data\papers\10048790.txtDIPinteraction1 82.57%Cdc42 cAMP Furthermore, PIX potentiates Cdc42 ac-several deletions in the N-terminus caused severe growth tivation of Pak and even enables a Cdc42 mutant that failsdefects and reduced the cAMP response to glucose feeding, to bind Pak to activate it [49].
...iorat\data\papers\10048790.txtDIPinteraction1ba 67.38%adenylyl cyclase many We suggest that the SH3 domains either cause hypermains have been identified through sequence comparisonsactivation of adenylyl cyclase or increase Ras binding to adand many ligand interactions have been studied by screen-enylyl cyclase.
...iorat\data\papers\10049744.txtDIPinteraction1 64.38%IFN IFN g and its receptor(31), it is unusual that IFN a and IFNb interact withthe same receptor chains but IFN
...iorat\data\papers\10049744.txtDIPinteraction1 5.94%IFNb a2 Furthermore, the presence of IFNb1b inthe immunoprecipitated IFN receptor complex suggests that IFNb interacts and binds differently to thereceptor than IFN
...iorat\data\papers\10049744.txtDIPinteraction1 40.98%IFNb IFNAR2 If dissociation of IFNb from the receptor complex didoccur then the IFNAR1/IFNAR2 complex would need to be held together largely by interactions between thereceptor chains themselves.
...iorat\data\papers\10049744.txtDIPinteraction1 65.91%IFNAR1 b1b IFN activity was detected in anti-IFNAR1 immunoprecipitates from Daudi cells stimulatedwith IFN
...iorat\data\papers\10049744.txtDIPinteraction1ba 70.8%IFN IFNAR1 aand IFN b may have similar receptor affinities (4,11),but assumes IFN
...iorat\data\papers\10049915.txtDIPinteraction1ba 50.68%rad53-31 CDC7 rad53-31 double mutants are viable because rad53-31onstrates a genetic interaction with CDC7.The rad53-11 ( 5mec2-1) is synthetically lethal with retains an intact checkpoint function.We reasoned that, because
...iorat\data\papers\10049915.txtDIPinteraction1a 61.74%rad53- rad53-31 the level of DBF4 message was increased in the rad53- protein expression in the rad53-31 and rad53-11 strainswas similar to that seen for mRNA expression.
...iorat\data\papers\10049915.txtDIPinteraction1ba 75.93%RAD53 DBF4 Second, RAD53 regu-lates the expression of DBF4 at the message and proteindominant but weak checkpoint signal from rad53-31.
...iorat\data\papers\10049915.txtDIPinteraction1ba 82.47%RNR1 RNR1 Genes of RNR1 because high-copy expression of RNR1 can Dev.
...iorat\data\papers\10049915.txtDIPinteraction1 53.59%rad53 Dbf4p bypass rad53D::URA3.Genetic interactions between rad53 and various cell cycle Interaction of Rad53p and Dbf4p: Given that RAD53 mutations were tested by crossing individual strains together.The diploids were sporulated and dissected and the resulting interacts genetically with CDC7 and DBF4, we asked if
...iorat\data\papers\10049915.txtDIPinteraction1 58.65%DBF4 DBF4 RAD53 regulates DBF4 expression at the mRNA andfirst complemented by wild-type DBF4 on a plasmid, protein levels: To understand how rad53-31 manifests
...iorat\data\papers\10049915.txtDIPinteraction1 81.72%URA3 Dbf4p tion that allows repeated use of URA3 selection in the construc-With the dramatic reduction of Dbf4p protein, the
...iorat\data\papers\10049915.txtDIPinteraction3 51.87%rad53-11 cdc7 The A. Interaction between rad53-11 and various cdc7 alleles results are complicated by the fact that strains bearingWild type (299) Viable (10 tetrads)
...iorat\data\papers\10049915.txtDIPinteraction1a 61.74%rad53- rad53-11 the level of DBF4 message was increased in the rad53- protein expression in the rad53-31 and rad53-11 strainswas similar to that seen for mRNA expression.
...iorat\data\papers\10049915.txtDIPinteraction1ba 55.36%Rad53p Dbf4p The results shownificant deviation from the 1PD:4T:1NPD ratio was observed that Rad53p interacts weakly with Dbf4p, but not with
...iorat\data\papers\10049915.txtDIPinteraction1 6.9%replication replication kinase complex is brought to origins of replication via
...iorat\data\papers\10049915.txtDIPinteraction1 67.7%Dbf4p Rad53p Longer exposures re-vealed a very low level of Dbf4p expression in the rad- tion of Rad53p, and the essential function of RAD53(Zheng et al. 1993).
...iorat\data\papers\10049915.txtDIPinteraction3 52.97%rad53-31 cdc8-1 Interaction between rad53-31 and cdc8-1 in the diploid.
...iorat\data\papers\10049915.txtDIPinteraction1b 2.18%Rad53p Dbf4p Two-hybrid analysis indicates that the Rad53p protein binds to Dbf4p.
...iorat\data\papers\10049915.txtDIPinteraction1 80.39%DBF4 RAD53 This work was supported by U.S. Public Health Service grant GM35078 awarded to R.A.S.In addition, the activation of DBF4 transcription and/
...iorat\data\papers\10049915.txtDIPinteraction1 60.96%bob1-1 rad53 A. bob1-1 or second site suppressor suppresses rad53D::URA3 mutationbob1/ 1 1/rad53D::URA3 4:0 viable:inviable 0(299 3 P119) 3:1 viable:inviable 9a2:2 viable:inviable 16
...iorat\data\papers\10049915.txtDIPinteraction1ba 89.46%epsilon replication epsilon links the DNA replication machinery to the S phase check-Donaldson, A. D., W. L. Fangman and B. J. Brewer, 1998 Cdc7 point.
...iorat\data\papers\10049915.txtDIPinteraction1b 11.75%Rad53p Dbf4p In addition, we show that Rad53p interacts with Dbf4p and controls the level of expression of DBF4.cell cycle and allow the completion of DNA repair orreplication (Sidorova and Breeden 1997).
...iorat\data\papers\10049915.txtDIPinteraction1ba 59.66%bob1-1 rad53 The bob1-1 mutation cannot suppress a rad53D::URA3 (Figure 3A).
...iorat\data\papers\10049915.txtDIPinteraction1 75.79%cdc7-1 Dbf4p of reduced activity of the cdc7-1 gene product and a interact directly through Dbf4p.
...iorat\data\papers\10049915.txtDIPinteraction3 53.26%rad53-31 dbf4-1 C. Interaction between rad53-31 and dbf4-1 suppressor was responsible for the suppression, notdbf4-1 (PDY029) Slow growth phenotype (31, 24 tetrads
...iorat\data\papers\10064605.txtDIPinteraction3 97.12%replication topoisomerase USA, 95, 6049-6054.Tsao,Y.P., Russo,A., Nyamusa,G., Silber,R. and Liu,L.F. (1993) Interaction between replication forks and topoisomerase I-DNAcleavable complexes: studies in a cell-free SV40 DNA replication system.
...iorat\data\papers\10064605.txtDIPinteraction1ba 16.64%topoisomerase RPA2 treated with topoisomerase inhibitors and ionizingradiations RPA2 phosphorylation was determined by Western blottingof cell lysates using specific monoclonal antibody.
...iorat\data\papers\10064605.txtDIPinteraction1 81.54%Rb p21 1 phase accumulation induced by UCN-01 isassociated with dephosphorylation of Rb and CDK2 proteins as well
...iorat\data\papers\10064605.txtDIPinteraction1 92.02%p53 p53 Miller,S.D., Moses,K., Jayaraman,L. and Prives,C. (1997) Complexformation between p53 and replication protein A inhibits the sequencespecific DNA binding of p53 and is regulated by single-strandedDNA.
...iorat\data\papers\10064605.txtDIPinteraction2 26.76%RPA2 protein kinase Inhibition of RPA2 phosphorylation and DNA-PKactivation by the protein kinase inhibitors wortmannin and UCN-01Next, we used wortmannin to obtain further evidence for a role of DNA-PK in RPA2 phosphorylation.
...iorat\data\papers\10064605.txtDIPinteraction1 92.68%p34 Cdc2 , 57, 3386-3389.Niu,H., Erdjument-Bromage,H., Pan,Z.Q., Lee,S.H., Tempst,P. and Hurwitz,J. (1997) Mapping of amino acid residues in the p34 subunitof human single-stranded DNA-binding protein phosphorylated by DNA-dependent protein kinase and Cdc2 kinase in vitro.
...iorat\data\papers\10064605.txtDIPinteraction1 85.45%cdc2 replication , 4, 968-977.Dutta,A. and Stillman,B. (1992) cdc2 family kinases phosphorylate a human cell DNA replication factor, RPA and activate DNA replication.EMBO J., 11, 2189-2199.