hspred : index

HSPred: Predictions of Hot Spot Residues at Protein-Protein Interfaces
Protein-protein interactions are critically dependent on just a few 'hot spot' residues at the interface. Hot spots make a dominant contribution to the free energy of binding and can disrupt the interaction if mutated to alanine. HSPred is a computational method to predict hot spot residues at protein-protein interfaces. It is based on Support Vector Machines (SVM) and and on calculated energy potentials. It relies on the structure of the complex as input. Hot spots are here defined as those residues for which Δ Δ G > 2 kcal/mol (where Δ Δ G is the change in binding free energy due to an alanine mutation).

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Interface Definition
List chain identifiers for the proteins on either face of the interface to be analysed. Use capital letters, space separate entries (e.g. "A B"). Protein 1: Protein 2: If using the test pdb file linked above: input "A" for 'Protein 1' and input "B" for 'Protein 2'.


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